| pEX18Tc pEX-18Tc 自殺質粒 | ||||||||||||||||||||||
| 發布時間:2018-05-28 12:01:53 | 瀏覽次數： | ||||||||||||||||||||||
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出品公司: |
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載體名稱: |
pEX18Tc, pEX-18Tc |
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質粒類型: |
自殺質粒 |
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高拷貝/低拷貝: |
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克隆方法: |
限制性內切酶，多克隆位點 |
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啟動子: |
Lac |
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載體大小: |
6349 bp |
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5' 測序引物及序列: |
M13F(-47) CGCCAGGGTTTTCCCAGTCACGAC |
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3' 測序引物及序列: |
M13R(-48) AGCGGATAACAATTTCACACAGGA |
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載體抗性: |
四環素 |
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病毒/非病毒: |
非病毒 |
1 TCGCGCGTTT CGGTGATGAC GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA
61 CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG
121 TTGGCGGGTG TCGGGGCTGG CTTAACTATG CGGCATCAGA GCAGATTGTA CTGAGAGTGC
181 ACCATAATCG GCATTTTCTT TTGCGTTTTT ATTTGTTAAC TGTTAATTGT CCTTGTTCAA
241 GGATGCTGTC TTTGACAACA GATGTTTTCT TGCCTTTGAT GTTCAGCAGG AAGCTAGGCG
301 CAAACGTTGA TTGTTTGTCT GCGTAGAATC CTCTGTTTGT CATATAGCTT GTAATCACGA
361 CATTGTTTCC TTTCGCTTGA GGTACAGCGA AGTGTGAGTA AGTAAAGGTT ACATCGTTAG
421 GATCAAGATC CATTTTTAAC ACAAGGCCAG TTTTGTTCAG CGGCTTGTAT GGGCCAGTTA
481 AAGAATTAGA AACATAACCA AGCATGTAAA TATCGTTAGA CGTAATGCCG TCAATCGTCA
541 TTTTTGATCC GCGGGAGTCA GTGAACAGAT ACCATTTGCC GTTCATTTTA AAGACGTTCG
601 CGCGTTCAAT TTCATCTGTT ACTGTGTTAG ATGCAATCAG CGGTTTCATC ACTTTTTTCA
661 GTGTGTAATC ATCGTTTAGC TCAATCATAC CGAGAGCGCC GTTTGCTAAC TCAGCCGTGC
721 GTTTTTTATC GCTTTGCAGA AGTTTTTGAC TTTCTTGACG GAAGAATGAT GTGCTTTTGC
781 CATAGTATGC TTTGTTAAAT AAAGATTCTT CGCCTTGGTA GCCATCTTCA GTTCCAGTGT
841 TTGCTTCAAA TACTAAGTAT TTGTGGCCTT TATCTTCTAC GTAGTGAGGA TCTCTCAGCG
901 TATGGTTGTC GCCTGAGCTG TAGTTGCCTT CATCGATGAA CTGCTGTACA TTTTGATACG
961 TTTTTCCGTC ACCGTCAAAG ATTGATTTAT AATCCTCTAC ACCGTTGATG TTCAAAGAGC
1021 TGTCTGATGC TGATACGTTA ACTTGTGCAG TTGTCAGTGT TTGTTTGCCG TAATGTTTAC
1081 CGGAGAAATC AGTGTAGAAT AAACGGATTT TTCCGTCAGA TGTAAATGTG GCTGAACCTG
1141 ACCATTCTTG TGTTTGGTCT TTTAGGATAG AATCATTTGC ATCGAATTTG TCGCTGTCTT
1201 TAAAGACGCG GCCAGCGTTT TTCCAGCTGT CAATAGAAGT TTCGCCGACT TTTTGATAGA
1261 ACATGTAAAT CGATGTGTCA TCCGCATTTT TAGGATCTCC GGCTAATGCA AAGACGATGT
1321 GGTAGCCGTG ATAGTTTGCG ACAGTGCCGT CAGCGTTTTG TAATGGCCAG CTGTCCCAAA
1381 CGTCCAGGCC TTTTGCAGAA GAGATATTTT TAATTGTGGA CGAATCGAAC TCAGGAACTT
1441 GATATTTTTC ATTTTTTTGC TGTTCAGGGA TTTGCAGCAT ATCATGGCGT GTAATATGGG
1501 AAATGCCGTA TGTTTCCTTA TATGGCTTTT GGTTCGTTTC TTTCGCAAAC GCTTGAGTTG
1561 CGCCTCCTGC CAGCAGTGCG GTAGTAAAGG TTAATACTGT TGCTTGTTTT GCAAACTTTT
1621 TGATGTTCAT CGTTCATGTC TCCTTTTTTA TGTACTGTGT TAGCGGTCTG CTTCTTCCAG
1681 CCCTCCTGTT TGAAGATGGC AAGTTAGTTA CGCACAATAA AAAAAGACCT AAAATATGTA
1741 AGGGGTGACG CCAAAGTATA CACTTTGCCC TTTACACATT TTAGGTCTTG CCTGCTTTAT
1801 CAGTAACAAA CCCGCGCGAT TTACTTTTCG ACCTCATTCT ATTAGACTCT CGTTTGGATT
1861 GCAACTGGTC TATTTTCCTC TTTTGTTTGA TAGAAAATCA TAAAAGGATT TGCAGACTAC
1921 GGGCCTAAAG AACTAAAAAA TCTATCTGTT TCTTTTCATT CTCTGTATTT TTTATAGTTT
1981 CTGTTGCATG GGCATAAAGT TGCCTTTTTA ATCACAATTC AGAAAATATC ATAATATCTC
2041 ATTTCACTAA ATAATAGTGA ACGGCAGGTA TATGTGATGG GTTAAAAAGG ATCGATCCTC
2101 TAGCTAGAGT CGATCTTCGC CAGCAGGGCG AGGATCGTGG CATCACCGAA CCGCGCCGTG
2161 CGCGGGTCGT CGGTGAGCCA GAGTTTCAGC AGGCCGCCCA GGCGGCCCAG GTCGCCATTG
2221 ATGCGGGCCA GCTCGCGGAC GTGCTCATAG TCCACGACGC CCGTGATTTT GTAGCCCTGG
2281 CCGACGGCCA GCAGGTAGGC CGACAGGCTC ATGCCGGCCG CCGCCGCCTT TTCCTCAATC
2341 GCTCTTCGTT CGTCTGGAAG GCAGTACACC TTGATAGGTG GGCTGCCCTT CCTGGTTGGC
2401 TTGGTTTCAT CAGCCATCCG CTTGCCCTCA TCTGTTACGC CGGCGGTAGC CGGCCAGCCT
2461 CGCAGAGCAG GATTCCCGTT GAGCACCGCC AGGTGCGAAT AAGGGACAGT GAAGAAGGAA
2521 CACCCGCTCG CGGGTGGGCC TACTTCACCT ATCCTGCCCG GCTGACGCCG TTGGATACAC
2581 CAAGGAAAGT CTACACGAAC CCTTTGGCAA AATCCTGTAT ATCGTGCGAA AAAGGATGGA
2641 TATACCGAAA AAATCGCTAT AATGACCCCG AAGCAGGGTT ATGCAGCGGA AAAGCGCTGC
2701 TTCCCTGCTG TTTTGTGGAA TATCTACCGA CTGGAAACAG GCAAATGCAG GAAATTACTG
2761 AACTGAGGGG ACAGGCGAGA GACGATGCCA AAGAGCTACA CCGACGAGCT GGCCGAGTGG
2821 GTTGAATCCC GCGCGGCCAA GAAGCGCCGG CGTGATGAGG CTGCGGTTGC GTTCCTGGCG
2881 GTGAGGGCGG ATGTCGATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG GAGAAAATAC
2941 CGCATCAGGC GCATATTTGA ATGTATTTAG AAAATAAACA AAAGAGTTTG TAGAAACGCA
3001 AAAAGGCCAT CCGTCAGGAT GGCCTTCTGC TTAATTTGAT GCCTGGCAGT TTATGGCGGG
3061 CGTCCTGCCC GCCACCCTCC GGGCCGTTGC TTCGCAACGT TCAAATCCGC TCCCGGCGGA
3121 TTTGTCCTAC TCAGGAGAGC GTTCACCGAC AAACAACAGA TAAAACGAAA GGCCCAGTCT
3181 TTCGACTGAG CCTTTCGTTT TATTTGATGC CTGGCAGTTC CCTACTCTCG CATGGGGAGA
3241 CCCCACACTA CCATCGGCGC TACGGCGTTT CACTTCTGAG TTCGGCATGG GGTCAGGTGG
3301 GACCACCGCG CTACTGCCGC CAGGCAAATT CTGTTTTATC AGACCGCTTC TGCGTTCTGA
3361 TTTAATCTGT ATCAGGCTGA AAATCTTCTC TCATCCGCCA AAACAGCCAA GCTCGCCATT
3421 CGCCATTCAG GCTGCGCAAC TGTTGGGAAG GGCGATCGGT GCGGGCCTCT TCGCTATTAC
3481 GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA GGCGATTAAG TTGGGTAACG CCAGGGTTTT
3541 CCCAGTCACG ACGTTGTAAA ACGACGGCCA GTGCCAAGCT TGCATGCCTG CAGGTCGACT
3601 CTAGAGGATC CCCGGGTACC GAGCTCGAAT TCGTAATCAT GGTCATAGCT GTTTCCTGTG
3661 TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA
3721 GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG CGTTGCGCTC ACTGCCCGCT
3781 TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA
3841 GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA CTGACTCGCT GCGCTCGGTC
3901 GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA
3961 TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT
4021 AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA
4081 AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT
4141 CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG
4201 TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC
4261 AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC
4321 GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA
4381 TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT
4441 ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGGACAGT ATTTGGTATC
4501 TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA
4561 CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA
4621 AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA
4681 AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT
4741 TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAAAC TTGGTCTGAC
4801 AATCGATGCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA
4861 TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC
4921 CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC
4981 AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG
5041 CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG
5101 ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC
5161 GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA
5221 GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG
5281 TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC
5341 AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA
5401 GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC
5461 GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG
5521 CATCGGTCGG CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT
5581 GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGT AAGGAGATGG CGCCCAACAG
5641 TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA
5701 GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC
5761 TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGAATCCACA GGACGGGTGT
5821 GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG
5881 GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC
5941 ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG
6001 CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT
6061 GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA
6121 ATTTAACTGT GATAAACTAC CGCATTAAAG CTAATCGGTT GAATACTCAT ACTCTTCCTT
6181 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA
6241 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT
6301 GACGTCTAAG AAACCATTAT TATCATGACA TTAACCTATA AAAATAGGCG TATCACGAGG
6361 CCCTTTCGTC
