測序及更多信息： https://pan.baidu.com/s/1Y8L1PLhPGl3S96R4ZyT1FQ?pwd=hedg
采購地址：淘寶店鋪地址
名稱:
貨號、類型:
規格:
別名:
啟動子:
復制子:
克隆菌株:
培養條件:
質粒宿主：
質粒用途：
插入類型：
物種：
原核抗性：
篩選標記：
熒光標記：
質粒類型: 慢病毒載體包裝載體
搭配質粒：pSPAX2
高拷貝/低拷貝: 高拷貝
啟動子: CMV
克隆方法: 多克隆位點，限制性內切酶
載體大小: 5824 bp
5' 測序引物及序列: CMV Fwd: CGCAAATGGGCGGTAGGCGTG
載體抗性: Ampicillin (氨芐青霉素)
備注: Envelope plasmid. Known to work with
most Aebischer and Trono lab lentiviral vectors, as well as the pLKO system
全序列
1 GGATCCCCTG AGGGGGCCCC CATGGGCTAG AGGATCCGGC CTCGGCCTCT GCATAAATAA
61 AAAAAATTAG TCAGCCATGA GCTTGGCCCA TTGCATACGT TGTATCCATA TCATAATATG
121 TACATTTATA TTGGCTCATG TCCAACATTA CCGCCATGTT GACATTGATT ATTGACTAGT
181 TATTAATAGT AATCAATTAC GGGGTCATTA GTTCATAGCC CATATATGGA GTTCCGCGTT
241 ACATAACTTA CGGTAAATGG CCCGCCTGGC TGACCGCCCA ACGACCCCCG CCCATTGACG
301 TCAATAATGA CGTATGTTCC CATAGTAACG CCAATAGGGA CTTTCCATTG ACGTCAATGG
361 GTGGAGTATT TACGGTAAAC TGCCCACTTG GCAGTACATC AAGTGTATCA TATGCCAAGT
421 ACGCCCCCTA TTGACGTCAA TGACGGTAAA TGGCCCGCCT GGCATTATGC CCAGTACATG
481 ACCTTATGGG ACTTTCCTAC TTGGCAGTAC ATCTACGTAT TAGTCATCGC TATTACCATG
541 GTGATGCGGT TTTGGCAGTA CATCAATGGG CGTGGATAGC GGTTTGACTC ACGGGGATTT
601 CCAAGTCTCC ACCCCATTGA CGTCAATGGG AGTTTGTTTT GGCACCAAAA TCAACGGGAC
661 TTTCCAAAAT GTCGTAACAA CTCCGCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG
721 TGGGAGGTCT ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGATCGCCTG GAGACGCCAT
781 CCACGCTGTT TTGACCTCCA TAGAAGACAC CGGGACCGAT CCAGCCTCCC CTCGAAGCTT
841 ACATGTGGTA CCGAGCTCGG ATCCTGAGAA CTTCAGGGTG AGTCTATGGG ACCCTTGATG
901 TTTTCTTTCC CCTTCTTTTC TATGGTTAAG TTCATGTCAT AGGAAGGGGA GAAGTAACAG
961 GGTACACATA TTGACCAAAT CAGGGTAATT TTGCATTTGT AATTTTAAAA AATGCTTTCT
1021 TCTTTTAATA TACTTTTTTG TTTATCTTAT TTCTAATACT TTCCCTAATC TCTTTCTTTC
1081 AGGGCAATAA TGATACAATG TATCATGCCT CTTTGCACCA TTCTAAAGAA TAACAGTGAT
1141 AATTTCTGGG TTAAGGCAAT AGCAATATTT CTGCATATAA ATATTTCTGC ATATAAATTG
1201 TAACTGATGT AAGAGGTTTC ATATTGCTAA TAGCAGCTAC AATCCAGCTA CCATTCTGCT
1261 TTTATTTTAT GGTTGGGATA AGGCTGGATT ATTCTGAGTC CAAGCTAGGC CCTTTTGCTA
1321 ATCATGTTCA TACCTCTTAT CTTCCTCCCA CAGCTCCTGG GCAACGTGCT GGTCTGTGTG
1381 CTGGCCCATC ACTTTGGCAA AGCACGTGAG ATCTGAATTC TGACACTATG AAGTGCCTTT
1441 TGTACTTAGC CTTTTTATTC ATTGGGGTGA ATTGCAAGTT CACCATAGTT TTTCCACACA
1501 ACCAAAAAGG AAACTGGAAA AATGTTCCTT CTAATTACCA TTATTGCCCG TCAAGCTCAG
1561 ATTTAAATTG GCATAATGAC TTAATAGGCA CAGCCATACA AGTCAAAATG CCCAAGAGTC
1621 ACAAGGCTAT TCAAGCAGAC GGTTGGATGT GTCATGCTTC CAAATGGGTC ACTACTTGTG
1681 ATTTCCGCTG GTATGGACCG AAGTATATAA CACAGTCCAT CCGATCCTTC ACTCCATCTG
1741 TAGAACAATG CAAGGAAAGC ATTGAACAAA CGAAACAAGG AACTTGGCTG AATCCAGGCT
1801 TCCCTCCTCA AAGTTGTGGA TATGCAACTG TGACGGATGC CGAAGCAGTG ATTGTCCAGG
1861 TGACTCCTCA CCATGTGCTG GTTGATGAAT ACACAGGAGA ATGGGTTGAT TCACAGTTCA
1921 TCAACGGAAA ATGCAGCAAT TACATATGCC CCACTGTCCA TAACTCTACA ACCTGGCATT
1981 CTGACTATAA GGTCAAAGGG CTATGTGATT CTAACCTCAT TTCCATGGAC ATCACCTTCT
2041 TCTCAGAGGA CGGAGAGCTA TCATCCCTGG GAAAGGAGGG CACAGGGTTC AGAAGTAACT
2101 ACTTTGCTTA TGAAACTGGA GGCAAGGCCT GCAAAATGCA ATACTGCAAG CATTGGGGAG
2161 TCAGACTCCC ATCAGGTGTC TGGTTCGAGA TGGCTGATAA GGATCTCTTT GCTGCAGCCA
2221 GATTCCCTGA ATGCCCAGAA GGGTCAAGTA TCTCTGCTCC ATCTCAGACC TCAGTGGATG
2281 TAAGTCTAAT TCAGGACGTT GAGAGGATCT TGGATTATTC CCTCTGCCAA GAAACCTGGA
2341 GCAAAATCAG AGCGGGTCTT CCAATCTCTC CAGTGGATCT CAGCTATCTT GCTCCTAAAA
2401 ACCCAGGAAC CGGTCCTGCT TTCACCATAA TCAATGGTAC CCTAAAATAC TTTGAGACCA
2461 GATACATCAG AGTCGATATT GCTGCTCCAA TCCTCTCAAG AATGGTCGGA ATGATCAGTG
2521 GAACTACCAC AGAAAGGGAA CTGTGGGATG ACTGGGCACC ATATGAAGAC GTGGAAATTG
2581 GACCCAATGG AGTTCTGAGG ACCAGTTCAG GATATAAGTT TCCTTTATAC ATGATTGGAC
2641 ATGGTATGTT GGACTCCGAT CTTCATCTTA GCTCAAAGGC TCAGGTGTTC GAACATCCTC
2701 ACATTCAAGA CGCTGCTTCG CAACTTCCTG ATGATGAGAG TTTATTTTTT GGTGATACTG
2761 GGCTATCCAA AAATCCAATC GAGCTTGTAG AAGGTTGGTT CAGTAGTTGG AAAAGCTCTA
2821 TTGCCTCTTT TTTCTTTATC ATAGGGTTAA TCATTGGACT ATTCTTGGTT CTCCGAGTTG
2881 GTATCCATCT TTGCATTAAA TTAAAGCACA CCAAGAAAAG ACAGATTTAT ACAGACATAG
2941 AGATGAACCG ACTTGGAAAG TAACTCAAAT CCTGCACAAC AGATTCTTCA TGTTTGGACC
3001 AAATCAACTT GTGATACCAT GCTCAAAGAG GCCTCAATTA TATTTGAGTT TTTAATTTTT
3061 ATGGAATTCA CCCCACCAGT GCAGGCTGCC TATCAGAAAG TGGTGGCTGG TGTGGCTAAT
3121 GCCCTGGCCC ACAAGTTTCA CTAAGCTCGC TTCCTTGCTG TCCAATTTCT ATTAAAGGTT
3181 CCTTGGTTCC CTAAGTCCAA CTACTAAACT GGGGGATATT ATGAAGGGCC TTGAGCATCT
3241 GGATTCTGCC TAATAAAAAA CATTTATTTT CATTGCAATG ATGTATTTAA ATTATTTCTG
3301 AATATTTTAC TAAAAAGGGA ATGTGGGAGG TCAGTGCATT TAAAACATAA AGAAATGAAG
3361 AGCTAGTTCA AACCTTGGGA AAATACACTA TATCTTAAAC TCCATGAAAG AAGGTGAGGC
3421 TGCAAACAGC TAATGCACAT TGGCAACAGC CCTGATGCCT ATGCCTTATT CATCCCTCAG
3481 AAAAGGATTC AAGTAGAGGC TTGATTTGGA GGTTAAAGTT TGGCTATGCT GTATTTTACA
3541 TTACTTATTG TTTTAGCTGT CCTCATGAAT GTCTTTTCAC TACCCATTTG CTTATCCTGC
3601 ATCTCTCAGC CTTGACTCCA CTCAGTTCTC TTGCTTAGAG ATACCACCTT TCCCCTGAAG
3661 TGTTCCTTCC ATGTTTTACG GCGAGATGGT TTCTCCTCGC CTGGCCACTC AGCCTTAGTT
3721 GTCTCTGTTG TCTTATAGAG GTCTACTTGA AGAAGGAAAA ACAGGGGGCA TGGTTTGACT
3781 GTCCTGTGAG CCCTTCTTCC CTGCCTCCCC CACTCACAGT GACCCGGAAT CCCTCGACAT
3841 GGCAGTCTAG CACTAGTGCG GCCGCAGATC TGCTTCCTCG CTCACTGACT CGCTGCGCTC
3901 GGTCGTTCGG CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC
3961 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA
4021 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA
4081 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC
4141 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA
4201 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CAATGCTCAC GCTGTAGGTA
4261 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA
4321 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA
4381 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG
4441 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG
4501 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG
4561 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG
4621 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA
4681 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT
4741 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC
4801 TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC
4861 ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC
4921 TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC
4981 AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC
5041 CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT
5101 GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC
5161 TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA
5221 AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT
5281 ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG
5341 CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC
5401 GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA
5461 AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT
5521 GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT
5581 CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG
5641 GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA
5701 TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT
5761 AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GT
