• Purpose

  hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on Hyg

Backbone

• Vector backbone
  Episomal ARS/CEN Backbone
• Vector type
  Yeast Expression, CRISPR
• Selectable markers
  Hygromycin

Growth in Bacteria

• Bacterial Resistance(s)
  Hygromycin, 200 μg/mL
• Growth Temperature
  37°C
• Growth Strain(s)
  DH5alpha
• Copy number
  High Copy

參考文獻

CRISPR/Cas9-Mediated Homology-Directed Genome Editing in Pichia pastoris. Gassler T, Heistinger L, Mattanovich D, Gasser B, Prielhofer R. Methods Mol Biol. 2019;1923:211-225. doi: 10.1007/978-1-4939-9024-5_9. 10.1007/978-1-4939-9024-5_9 PubMed 30737742